陈春来 博士

副教授

1999-2003      北京大学化学与分子工程学院        学士

2003-2008     北京大学化学与分子工程学院         博士

2008-2015      University of Pennsylvania             博士后

2015-2018      yh533388银河                   助理教授

2018-至今       yh533388银河                   副教授

 

研究兴趣、领域

1) 发展新型单分子荧光技术；

2) 蛋白在DNA上的靶向搜寻机制的研究；

3) CRISPR系列蛋白靶向和剪切机制的研究。

 

代表性论文

1.  Yuan Zhang (#), Chen Yang (#), Sijia Peng, Jing Ling, Peng Chen, Yumiao Ma, Wenjuan Wang, Zhixing Chen (*), Chunlai Chen (*), (2023) General strategy to improve the photon budget of thiol-conjugated cyanine dyes, Journal of the American Chemical Society, 145, 7, 4187-4198

2.  Jie Heng, Yunfei Hu, Guillermo Pérez-Hernández, Asuka Inoue, Jiawei Zhao, Xiuyan Ma, Xiaoou Sun, Kouki Kawakami, Tatsuya Ikuta, Jienv Ding, Yujie Yang, Lujia Zhang, Sijia Peng, Xiaogang Niu, Hongwei Li, Ramon Guixà-González, Changwen Jin, Peter W Hildebrand, Chunlai Chen (*), Brian K. Kobilka (*), (2023) Function and dynamics of the intrinsically disordered carboxyl terminus of β2 adrenergic receptor, Nat Commun, Accepted

3.  Yirong Yao, Wenjuan Wang, Chunlai Chen (*), (2022) Mechanisms of phase-separation-induced cGAS activation revealed by dcFCCS, PNAS Nexus, doi.org/10.1093/pnasnexus/pgac109

4.  Jingfei Xu(#), Lingyun Zhao(#), Sijia Peng(#), Huiying Chu(#), Rui Liang, Meng Tian, Philip P. Connell, Guohui Li (*), Chunlai Chen (*) and Hong-Wei Wang  (*), (2021) Mechanisms of distinctive mismatch tolerance between Rad51 and Dmc1 in homologous recombination, Nucleic Acids Research, 49, 13135-13149

5.  Xiaolin Niu(#), Ruirui Sun(#), Zhifeng Chen, Yirong Yao, Xiaobing Zuo, Chunlai Chen (*) and Xianyang Fang (*), (2021) Pseudoknot length modulates the folding, conformational dynamics and robustness of Xrn1 resistance of flaviviral xrRNAs, Nat Commun, 12, 6417

6.  Mengyi Yang, Ruirui Sun, Pujuan Deng, Yuzhuo Yang, Wenjuan Wang, Jun-Jie Gogo Liu (*) and Chunlai Chen (*), (2021) Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search, Chem Sci, 12, 12776-12784

7.  Victor Pan, Wen Wang, Ian Heaven, Tanxi Bai, Yongxin Cheng, Chunlai Chen (*), Yonggang Ke (*) and Bryan Wei (*), (2021) Monochromatic Fluorescent Barcodes Hierarchically Assembled from Modular DNA Origami Nanorods, ACS Nano, 15, 15892–15901

8.  Ming-Jie Han(#), Qing-tao He(#), Mengyi Yang(#), Chao Chen, Yirong Yao, Xiaohong Liu, Yuchuan Wang, Zhong-liang Zhu, Kong-kai Zhu, Changxiu Qu, Fan Yang, Cheng Hu, Xuzhen Guo, Dawei Zhang, Chunlai Chen (*), Jin-peng Sun (*) and  Jiangyun Wang (*), (2021) Single-molecule FRET and conformational analysis of beta-arrestin-1 through genetic code expansion and a Se-click reaction, Chem Sci, 12, 9114-9123

9.  Sijia Peng (#), Weiping Li (#), Yirong Yao (#), Wenjing Xing, Pilong Li (*) and Chunlai Chen (*), (2020) Phase separation at the nanoscale quantified by dcFCCS, PNAS, 117(44):27124-27131

10.  Sijia Peng (#), Xiaowei Wang (#), Lu Zhang (#)(*), Shanshan He, Xin Sheng Zhao (*), Xuhui Huang (*) and Chunlai Chen (*), (2020) Target search and recognition mechanisms of glycosylase AlkD revealed by scanning FRET-FCS and Markov State Models, PNAS, 117(36):21889-21895

11.  Lujia Zhang, Ruirui Sun, Mengyi Yang, Sijia Peng, Yongxin Cheng, and Chunlai Chen (*), (2019) Conformational dynamics and cleavage sites of Cas12a are modulated by complementarity between crRNA and DNA, iScience, 19:492-503

12.  Man Pan (#), Qingyun Zheng (#), Shan Ding (#), Lujia Zhang (#), Qian Qu, Tian Wang, Danning Hong, Yunjing Ren, Lujun Liang, Chunlai Chen (*), Ziqing Mei (*), and Lei Liu (*), (2019) Chemical Protein Synthesis-Enabled Mechanistic Studies on the Molecular Recognition of K27-linked Ubiquitin Chains, Angew. Chem. Int. Ed, 58(9):2627-2631

13.  Bo Wu, Haibo Zhang, Ruirui Sun, Sijia Peng, Barry S Cooperman, Yale E Goldman (*), and Chunlai Chen (*), (2018) Translocation kinetics and structural dynamics of ribosomes are modulated by the conformational plasticity of downstream pseudoknots, Nucleic Acids Research, 46(18): 9736-9748

14.  Mengyi Yang(#), Sijia Peng(#), Ruirui Sun, Jingdi Lin, Nan Wang, and Chunlai Chen(*), (2018) The Conformational Dynamics of Cas9 Governing DNA Cleavage Are Revealed by Single-Molecule FRET, Cell Reports, 22(2): 372-382

15.  Sijia Peng, Ruirui Sun, Wenjuan Wang and Chunlai Chen(*), (2017) Single-Molecule Photoactivation FRET: A General and Easy-to-Implement Approach to Break the Concentration Barrier, Angew. Chem. Int. Ed, 56(24):6882-6885

 

联系方式

邮箱：chunlai@tsinghua.edu.cn